解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::We report a novel, highly sensitive and selective method for the detection and quantification of acyl-CoA esters from Arabidopsis thaliana. Extracted acyl-CoA esters were derived to their fluorescent acyl-etheno-CoA esters, separated by ion-paired reversed-phase HPLC, and detected fluorometrically. We report the compo...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Crystal structure data for Escherichia coli beta-ketoacyl synthase (KAS) I with C(10) and C(12) fatty acid substrates bound in conjunction with results from mutagenizing residues in the active site leads to a model for catalysis. Differences from and similarities to the other Claisen enzymes carrying out decarboxylati...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/0300-5127:0280601
更新日期:2000-12-01 00:00:00
abstract::From a random transposon mutagenesis experiment, using Petunia line W138, a seed-specific linoleic acid mutant was isolated. The tagged gene was cloned and identified as a microsomal Delta(12) desaturase. Expression of the gene, however, was constitutive and not, as might have been expected, seed-specific. Moreover, s...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::We have isolated a novel gene (GLELO) from Mortierella alpina and its homologue (CEELO1) from Caenorhabditis elegans and demonstrate the involvement of their encoded proteins in the elongation of C(18) polyunsaturated fatty acids. ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::The expression of diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) with predicted molecular mass of 56.9. kDa (BnDGAT1) was examined using microspore-derived cell suspension cultures of oilseed rape (Brassica napus L. cv Jet Neuf). As well, a recombinant histidine-tagged N-terminal fragment of BnDGAT1 [BnDGAT1((1-11...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::A comparative approach has been used to study the role of several lipid-body-binding proteins in plants and animals. Caleosins are a newly discovered class of calcium-binding lipid-body proteins found in plants and fungi, which we now report to have separate endoplasmic reticulum- and lipid-body-associated isoforms. W...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Many microalgae are known to accumulate triacylglycerols (TAGs), especially under nitrate starvation. Generally, these TAGs are predominantly constructed of saturated and monounsaturated fatty acids. In contrast, the TAGs of the red microalga Porphyridium cruentum are rich in arachidonic acid (AA, 20:4 omega6) and eic...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Genes encoding an alpha-oxygenase, in Nicotiana tabacum and Arabidopsis thaliana, have been recently isolated. However, the reaction mechanism of the enzyme has not so far been elucidated. In this study, a cDNA encoding the fatty acid alpha-oxygenase gene in rice plants was isolated. The deduced amino acid sequence sh...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Enzymes of the 1-deoxy-D-xylulose 5-phosphate/2-C-methylerythritol 4-phosphate (DOXP/MEP) pathway are targets for new herbicides and antibacterial drugs. Until now, no inhibitors for the DOXP synthase have been known of. We show that one of the breakdown products of the herbicide clomazone affects the DOXP synthase. O...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Stimulus-response coupling in animal cells frequently involves the hydrolysis of PtdIns(4,5)P(2) which is catalysed by phosphoinositide-specific phospholipase C (PI-PLC). There is an increasing body of evidence for PI-PLC-based signalling in plant cells; however, the physiological role of this system remains poorly do...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::The metabolism in vitro of [1-(14)C]linoleate, [1-(14)C]linolenate and their 9(S)-hydroperoxides in tulip (Tulipa gesneriana) was found to be under the control of 9-lipoxygenase and allene oxide synthase, and directed towards alpha-ketol, gamma-ketol and the novel compound (12Z)-10-oxo-11-hydroxy-12-octadecadienoic ac...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Diacylglyceryl-N,N,N-trimethylhomoserine (DGTS) belongs to a family of three known betaine lipids. It is synthesized by a number of bacteria, fungi, brown, red and green algae, in addition to lichens, and is also found in higher plants Bryophyta, Equisetophyta and Polydiophyta. It has been determined that DGTS plants ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Seeds of rape (Brassica napus L.) were germinated at various NaCl concentrations up to 200 mM. Germinating levels, seedling growth, triacylglycerol mobilization and lipase activity were investigated. High salt concentrations resulted in retardation of seed germination. Seedling growth as measured by radicle length was...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Post-transcriptional gene silencing (PTGS) has been successfully used to modify seed lipids in oilseed crops like soybean, canola and sunflower. Conventionally, PTGS has been induced by transforming the plants with either antisense or co-suppression constructs targeted against key seed lipid biosynthesis genes. A majo...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::One of the major goals of modern plant biotechnology is to manipulate lipid metabolism in oilseed crops to produce new and improved edible and industrial vegetable oils. Lipids constitute the structural components of cellular membranes and act as sources of energy for the germinating seed and are therefore essential t...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::African trypanosomes have plastic genomes with extensive variability at the chromosome ends. The genes encoding the expressed major surface protein of the infective bloodstream form stages of Trypanosoma brucei and are located at telomeres. These telomeric expression-site transcription units are turning out to be surp...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0280536
更新日期:2000-10-01 00:00:00
abstract::For a disease such as cancer, where a number of alterations to normal cell function accumulate over time, there are several opportunities to inhibit, slow down or even reverse the process. Many of the changes which drive the disease process occur in cell-signalling pathways that regulate proliferation and apoptosis. A...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0280007
更新日期:2000-02-01 00:00:00
abstract::An important aspect of the cytochrome c electrochemistry is the possibility of coupling the 'heterogeneous reactions' with other redox enzymes. Cellobiose dehydrogenase, a 89170 Da glycoprotein that contains both FAD and a b-type haem as prosthetic groups, donates electrons to a number of acceptors, including cytochro...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/bst0280063
更新日期:2000-02-01 00:00:00
abstract::In this study the network of ATP-consuming and -producing reactions, interacting via the cytosolic ATP/ADP+P(i) system, was studied for the first time in an intact organ, the isolated perfused rat liver, using top-down metabolic-control analysis. Flux control in the metabolically resting state (only oxidative phosphor...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/bst0280109
更新日期:2000-02-01 00:00:00
abstract::The mitochondrial outer membrane separates the intermembrane space from the cytosol. The whole exchange of metabolites, cations and information between mitochondria and the cell occurs through the outer membrane. Experimental evidence is reviewed supporting the hypothesis of dynamic ADP compartmentation within the int...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0280164
更新日期:2000-02-01 00:00:00
abstract::Human CD4 T helper lymphocytes can be subdivided into at least three distinct functional subsets on the basis of their cytokine secretion profiles. One type of CD4+ lymphocyte, T helper 1 (Th1), produces interferon (IFN)-gamma and tumour necrosis factor beta, a second type (Th2) produces interleukin (IL)-4 and IL-5 an...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0280212
更新日期:2000-02-01 00:00:00
abstract::A modification of the Structure Alignment Program (SAP), combined with a novel automatic method for the definition of structural elements, correctly identified the core folds of a variety of small beta/alpha proteins when compared with a series of ideal architectures. This approach opens the possibility of not just de...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/bst0280264
更新日期:2000-02-01 00:00:00
abstract::Growth and differentiation factor-5 (GDF-5) is a divergent member of the transforming growth factor-beta/bone morphogenetic protein (BMP) superfamily that is required for proper skeletal patterning and development in the vertebrate limb. Based on the homology of GDF-5 with other bone-inducing BMP family members, the i...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::We have shown that yeast mutants with defects in the Ada adaptor proteins are defective in hormone-dependent gene activation by ectopically expressed human glucocorticoid receptor (GR). Others have shown that the Ada2 protein is required for physical interactions between some activation domains and TBP (TATA-binding p...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::Invertebrates use a wide range of peptides as transmitters and hormones to regulate complex behaviour, physiology and development. These animals, especially those that are amenable to genetic study and are the subject of genome-sequencing projects, provide powerful model systems for understanding the functions of pept...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::A general strategy is described for the amplified expression, purification and characterization in Escherichia coli of multidrug efflux proteins from Staphylococcus aureus, Bacillus subtilis, Methanococcus janaschii and E. coli. They all catalyse drug/H(+) antiport of substrates such as quinolones and ethidium and exe...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Studies on those enzymes and electron-transfer proteins involved in the catabolism of 'C1' substrates in methylotrophic bacteria have provided a wealth of information concerning the transfer of electrons and hydrogen by quantum tunnelling mechanisms. With regard to H-transfer, studies with MADH have provided the first...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0270767
更新日期:1999-12-01 00:00:00
abstract::Three mammalian expression systems suitable for expressing recombinant receptors have been described. Each is suited to a different aspect of the study of receptors and their behaviour. IRES-based vectors are ideal for creating stable mammalian cell lines suitable for screening receptors using a signalling readout. Un...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0260699
更新日期:1998-11-01 00:00:00
abstract::CT responds to properties of PC-depleted membranes: increased negative charge density, which concentrates the enzyme at the membrane surface, and lipid packing perturbations, which create holes in the membrane surface into which the hydrophobic side chains of the amphipathic helix of domain M can intercalate. The PC-d...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0260539
更新日期:1998-08-01 00:00:00
abstract::It is clear that considerable conformational distortions may occur in peripheral proteins on interaction with anionic lipid bilayers. Specific lipid interactions do occur at least in the case of cytochrome c, and each perturbation and interaction may well take place before insertion into, or translocation across, a bi...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0251119
更新日期:1997-08-01 00:00:00
abstract::FcRn was originally identified as the receptor responsible for IgG binding to the intestinal epithelium of neonatal rats and mice. FcRn transports IgG from milk across the intestinal epithelial cells of the suckling animal. Subsequently, FcRn was detected in tissues involved in the transmission of IgG from mother to f...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0250481
更新日期:1997-05-01 00:00:00
abstract::The overall effects of oestrogen on the vasculature are beneficial as a result of its antioxidant properties and ability to enhance relaxation by modulating synthesis of endothelium-derived vasodilators and smooth-muscle cell Ca2+ signalling. Although the protective effects of oestrogen are well accepted, only limited...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0250040
更新日期:1997-02-01 00:00:00
abstract::The Cat family of genes have revealed unexpected complexity and regulation. The first 5 years of research have been productive, yet many important questions remain to be addressed. A major problem for the field of amino acid transport is a severe paucity of data on the structure of these proteins and their arrangement...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0240846
更新日期:1996-08-01 00:00:00
abstract::IAPP has been postulated to have a role as a modulating factor in glucose homoeostasis and to be involved in the pathophysiology of diabetes. However, the normal physiological functions of the peptide remain obscure: exogenous IAPP acts on many experimental systems to modulate nutrient supply and metabolism but there ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0240594
更新日期:1996-05-01 00:00:00
abstract::It has been clear for some time now that mucin glycoproteins have extensive, highly glycosylated tandem repeat domains. What is becoming increasingly apparent, however, is the diversity of unique sequences present on different mucins. Variations in mucin-unique sequences clearly result in major differences in physical...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0230795
更新日期:1995-11-01 00:00:00
abstract::Intracellular cross-talk or synergism between receptors coupled negatively to adenylate cyclase via PTX-sensitive Gi-protein(s) and receptors coupled to phospholipase C via PTX-insensitive G-proteins is prevalent in a variety of cell types (see above) and may represent a universal mechanism for the modulation of phosp...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0211124
更新日期:1993-11-01 00:00:00
abstract::We have described a large number of different mutations in the LPL gene that result in completely catalytically defective LPL protein. More recently exonic polymorphisms in the LPL gene have been described that do not result in the catalytic activity of LPL being significantly impaired. Furthermore we have recently de...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0210506
更新日期:1993-05-01 00:00:00
abstract::Large single crystals (up to 1 mm in each dimension) of the B800-850 antenna complex from Rhodopseudomonas acidophila strain 10050 have been grown in the presence of beta-octyl-glucoside. These crystals have the space group R32 and unit cell dimensions of a = b = 119.9 A and c = 297.0 A. Recently we have improved our ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/bst0210039
更新日期:1993-02-01 00:00:00
abstract::The examples shown here illustrate the power of gene targeting to the epidermis as a means of developing animal models for the study of human skin diseases. In this short review, I have focused on contributions which stem predominantly from my own laboratory [31, 32, 34, 37, 47]. However, other laboratories have also ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0191112
更新日期:1991-11-01 00:00:00
abstract::Genetic experiments have indicated that succinoglycan (EPS I), the acidic Calcofluor-binding exopolysaccharide, of the nitrogen-fixing bacterium Rhizobium meliloti strain Rm1021 is required for nodule invasion and possibly for later events in nodule development on alfalfa and other hosts. Fourteen exo loci on the seco...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0190636
更新日期:1991-08-01 00:00:00